RGS9-1 is required for normal inactivation of mouse cone phototransduction
CaltechAUTHORS
View Archive Info| Field | Value | |
| Title |
RGS9-1 is required for normal inactivation of mouse cone phototransduction |
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| Creator |
Lyubarsky, A. L.
Chen, C.-K. Naarendorp, F. Zhang, X. Wensel, T. Simon, M. I. Pugh, E. N., Jr. |
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| Subject |
Caltech Library Services
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| Description |
Purpose: To test the hypothesis that Regulator of G-protein Signaling 9 (RGS9-1) is necessary for the normal inactivation of retinal cones. Methods: Mice having the gene RGS9-1 inactivated in both alleles (RGS9-1 -/-) were tested between the ages 8-10 weeks with electroretinographic (ERG) protocols that isolate cone-driven responses. Immunohistochemistry was performed with a primary antibody against RGS9-1 (anti-RGS9-1c), with the secondary conjugated to fluorescein isothiocyanate, and with rhodamine-conjugated peanut agglutinin. Results: (1) Immunohistochemistry showed RGS9-1 to be strongly expressed in the cones of wildtype (WT is C57BL/6) mice, but absent from the cones of RGS9-1 mice. (2) Cone-driven b-wave responses of dark-adapted RGS9-1 -/- mice had saturating amplitudes and sensitivities in the midwave and UV regions of the spectrum equal to or slightly greater than those of WT (C57BL/6) mice. (3) Cone-driven b-wave and a-wave responses of RGS9-1 -/- mice recovered much more slowly than those of WT after a strong conditioning flash: for a flash estimated to isomerize 1.2% of the M-cone pigment and 0.9% of the UV-cone pigment, recovery of 50% saturating amplitude was approximately 60-fold slower than in WT. Conclusions: (1) The amplitudes and sensitivities of the cone-driven responses indicate that cones and cone-driven neurons in RGS9-1 -/- mice have normal generator currents. (2) The greatly retarded recovery of cone-driven responses of RGS9-1 -/- mice relative to those of WT mice establishes that RGS9-1 is required for normal inactivation of the cone phototransduction cascades of both UV- and M-cones. |
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| Date |
2001-03-20
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://authors.library.caltech.edu/265/1/LYUmv01.pdf
Lyubarsky, A. L. and Chen, C.-K. and Naarendorp, F. and Zhang, X. and Wensel, T. and Simon, M. I. and Pugh, E. N., Jr. (2001) RGS9-1 is required for normal inactivation of mouse cone phototransduction. Molecular Vision, 7 (11). pp. 71-78. ISSN 1090-0535. http://resolver.caltech.edu/CaltechAUTHORS:LYUmv01 <http://resolver.caltech.edu/CaltechAUTHORS:LYUmv01> |
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| Relation |
http://resolver.caltech.edu/CaltechAUTHORS:LYUmv01
http://authors.library.caltech.edu/265/ |
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