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RGS9-1 is required for normal inactivation of mouse cone phototransduction

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Title RGS9-1 is required for normal inactivation of mouse cone
phototransduction
 
Creator Lyubarsky, A. L.
Chen, C.-K.
Naarendorp, F.
Zhang, X.
Wensel, T.
Simon, M. I.
Pugh, E. N., Jr.
 
Subject Caltech Library Services
 
Description Purpose: To test the hypothesis that Regulator of G-protein Signaling 9 (RGS9-1) is necessary for the normal inactivation of retinal cones.
Methods: Mice having the gene RGS9-1 inactivated in both alleles (RGS9-1 -/-) were tested between the ages 8-10 weeks
with electroretinographic (ERG) protocols that isolate cone-driven responses. Immunohistochemistry was performed with a primary antibody against RGS9-1 (anti-RGS9-1c), with the secondary conjugated to fluorescein isothiocyanate, and
with rhodamine-conjugated peanut agglutinin.
Results: (1) Immunohistochemistry showed RGS9-1 to be strongly expressed in the cones of wildtype (WT is C57BL/6)
mice, but absent from the cones of RGS9-1 mice. (2) Cone-driven b-wave responses of dark-adapted RGS9-1 -/- mice had saturating amplitudes and sensitivities in the midwave and UV regions of the spectrum equal to or slightly greater than those of WT (C57BL/6) mice. (3) Cone-driven b-wave and a-wave responses of RGS9-1 -/- mice recovered much more slowly than those of WT after a strong conditioning flash: for a flash estimated to isomerize 1.2% of the M-cone pigment and 0.9% of the UV-cone pigment, recovery of 50% saturating amplitude was approximately 60-fold slower than in WT.
Conclusions: (1) The amplitudes and sensitivities of the cone-driven responses indicate that cones and cone-driven neurons in RGS9-1 -/- mice have normal generator currents. (2) The greatly retarded recovery of cone-driven responses of RGS9-1 -/- mice relative to those of WT mice establishes that RGS9-1 is required for normal inactivation of the cone
phototransduction cascades of both UV- and M-cones.
 
Date 2001-03-20
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://authors.library.caltech.edu/265/1/LYUmv01.pdf
Lyubarsky, A. L. and Chen, C.-K. and Naarendorp, F. and Zhang, X. and Wensel, T. and Simon, M. I. and Pugh, E. N., Jr. (2001) RGS9-1 is required for normal inactivation of mouse cone phototransduction. Molecular Vision, 7 (11). pp. 71-78. ISSN 1090-0535. http://resolver.caltech.edu/CaltechAUTHORS:LYUmv01 <http://resolver.caltech.edu/CaltechAUTHORS:LYUmv01>
 
Relation http://resolver.caltech.edu/CaltechAUTHORS:LYUmv01
http://authors.library.caltech.edu/265/